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The planeswalker's abilities each have a positive or negative loyalty cost; this is how many counters must be added (if positive) or removed (if negative) to play that ability. Abilities with negative loyalty costs may only be played if there are enough loyalty counters. Planeswalker Damage. Although they are not creatures, planeswalkers may be affected by damage. Whenever damage is dealt to a planeswalker, remove that many loyalty counters from it. A planeswalker with no loyalty counters, either through use of its abilities or through damage, is put into its owner's graveyard. There are two ways to deal damage to a planeswalker. If a player uses any spell or ability that would deal damage to an opponent, the player may instead choose to deal the damage to one of that opponent's planeswalkers. Note that planeswalkers are neither creatures nor players, so most spells and abilities cannot target them directly. Additionally, if a player attacks an opponent who controls a planeswalker, the player may declare any or all of the attacking creatures to be attacking the planeswalker instead. Those creatures may be blocked normally, but if not blocked deal damage to the planeswalker instead of the player. Planeswalkers count as Permanents. MTG - Magic: the Gathering; Return to Ravnica Spoiler; Return to Ravnica Set 1 of 3 in the Return to Ravnica block Number of Cards: 274 Prerelease Events: September. MTG - Magic: the Gathering; Journey into Nyx Spoiler; Set 3 of 3 in the Theros block Number of Cards: 165 Prerelease Events: April 26-27, 2014 Release Date: May 2. The New Bard's Handbook for Dungeons and Dragons 3.5, written by JoshuaD. A review of ability scores, races, prestige classes, feats, skills, skill tricks, spells. List of Known Planeswalkers. List of Planeswalker subtypes. It’s no secret that airline travel is more miserable than ever, with increasing lengths of time spent on the tarmac, “family fees,” lines to pass through. Ajani. 3. 06. Planeswalkers. A player who has priority may cast a planeswalker card from his or her hand during a main phase of his or her turn when the stack is empty. Casting a planeswalker as a spell uses the stack. See rule 6. 01, “Casting Spells.”). When a planeswalker spell resolves, its controller puts it onto the battlefield under his or her control. Planeswalker subtypes are always a single word and are listed after a long dash: “Planeswalker — Jace.” Each word after the dash is a separate subtype. Planeswalker subtypes are also called planeswalker types. Planeswalkers may have multiple subtypes. See rule 2. 05. 3j for the complete list of planeswalker types. If a player controls two or more planeswalkers that share a planeswalker type, that player chooses one of them, and the rest are put into their owners’ graveyards. This is called the “planeswalker uniqueness rule.” See rule 7. Loyalty is a characteristic only planeswalkers have. The loyalty of a planeswalker card not on the battlefield is equal to the number printed in its lower right corner. A planeswalker is treated as if its text box included, “This permanent enters the battlefield with a number of loyalty counters on it equal to its printed loyalty number.” This ability creates a replacement effect (see rule 6. ![]() The loyalty of a planeswalker on the battlefield is equal to the number of loyalty counters on it. Each planeswalker has a number of loyalty abilities, which are activated abilities with loyalty symbols in their costs. Loyalty abilities follow special rules: A player may activate a loyalty ability of a permanent he or she controls any time he or she has priority and the stack is empty during a main phase of his or her turn, but only if none of that permanent’s loyalty abilities have been activated that turn. See rule 6. 06, “Loyalty Abilities.”. Planeswalkers can be attacked. See rule 5. 08, “Declare Attackers Step.”). If noncombat damage would be dealt to a player by a source controlled by an opponent, that opponent may have that source deal that damage to a planeswalker the first player controls instead. This is a redirection effect (see rule 6. The opponent chooses whether to redirect the damage as the redirection effect is applied. Damage dealt to a planeswalker results in that many loyalty counters being removed from it. If a planeswalker’s loyalty is 0, it’s put into its owner’s graveyard. This is a state- based action. See rule 7. 04.). Game - Download - Dragon Raja. In order to play Dragon Raja Online, your computer must meet up to the following requirements: Minimum. Recommended. Pentium III 5. Mhz / AMD 5. 00. Pentium III 1. GHZ or Equivalent. MB RAM or higher. MB RAM or higher. GB or higher. 5GB or higher. Windows 2. 00. 0/XPWindows XP/Vista/7/8. MB or Higher. 32. MB or Higher. Direct. X 8. 00x. 60. 0 1. Bit color. Direct. X 1. 02. 4x. 76. 8 3. To use this software, your computer has to meet the following system requirements. 1. Supported OSes - Windows 8.1 (All 32-bit/64-bit systems).With the release of EOS Utility 2.14 and EOS Utility 3.0 Canon have changed the process for WiFi pairing your EOS camera with your computer. Canon has added an EOS. ![]() Canon eos utility free download - EOS Utility, Canon File Viewer Utility, Canon EOS 20D Firmware Update, and many more programs. View and Download Canon EOS 7D instruction manual online. EOS 7D Digital Camera pdf manual download. EOS Utility 2. 1. Updater for Windows. EOS Utility is an application that brings together all of the functions required to communicate with the camera. Functions for downloading and displaying images, remote shooting, and camera control of each setting when an EOS DIGITAL camera is connected to a computer have been integrated. Changes for EOS Utility 2. Updater for Windows . Supports EF3. 5mm f/1. L II USM, EF5. 0mm f/1. STM. To use this software, your computer has to meet the following system requirements. Supported OSes. - Windows 8. All 3. 2- bit/6. 4- bit systems). Windows 8 (All 3. Windows 7 (All 3. Starter Edition). Can also be used with Windows 1. Supported Computers. PCs with one of the supported OSes above preinstalled, that have USB ports as standard equipment. Operation is not guaranteed on machines that have been upgraded.). The . NET Framework 3. Microsoft's . NET Framework will be installed along with EOS Utility. Pentium 1. 3. GHz or faster. GB or greater. - 2 GB or greater (Windows 8. Windows 8 6. 4- bit / Windows 7 6. Target Applications. Make sure that at least one of the following applications is installed. EOS Viewer Utility. File Viewer Utility. Camera Window DSLR. Screen Resolution; 1,0. Screen colors; Medium (1. Supported Models. EOS 7. D Mark II, EOS Kiss X7. EOS REBEL T5 / EOS 1. D / EOS Hi, EOS M2, EOS 7. D, EOS Kiss X7 / EOS REBEL SL1 / EOS 1. D, EOS Kiss X7i / EOS REBEL T5i / EOS 7. D, EOS- 1. D C, EOS 6. D, EOS M, EOS 6. 0Da, EOS Kiss X6i / EOS REBEL T4i / EOS 6. D, EOS 5. D Mark III, EOS- 1. D X, EOS Kiss X5 / EOS REBEL T3i / EOS 6. D, EOS Kiss X5. 0 / EOS REBEL T3 / EOS 1. D, EOS 6. 0D, EOS Kiss X4 / EOS REBEL T2i / EOS 5. D, EOS 7. D, EOS- 1. D Mark IV, EOS Kiss X3 / EOS REBEL T1i / EOS 5. D, EOS 5. D Mark II, EOS 5. D, EOS 4. 0D, EOS Kiss F / EOS REBEL XS / EOS 1. D, EOS Kiss X2 / EOS REBEL XSi / EOS 4. D, EOS- 1. Ds Mark III, EOS- 1. D Mark IIIEOS Utility 2. Updater for Windows is an update program for EOS Utility and can be used only when. Your PC has a previous version of the software installed. Your PC has the CD- ROM inserted and recognizes a previous version of the software contained on the CD. When used with the EOS- 1. D X, the My Menu settings function is unavailable. Please use the camera's menu for the My Menu settings function. If you are using Windows Vista, Windows XP, use [EOS Utility 2. Please refer to the instructions below on how to download and install the software. Exit all other applications when installing this software. Make sure that at least one of the following applications is installed. EOS Viewer Utility. File Viewer Utility. Camera Window DSLR. Download "euw. 2. Save the "euw. 2. When the "euw. 2. Installation for EOS Utility will begin. If the User Account Control window appears, follow the on- screen instructions to proceed.). Follow the on- screen instructions to complete the installation. After the installation is complete, the EOS Utility installer may ask to restart the computer. In this case, restart the computer. If the installation finished properly, the downloaded file and the "euw. File name : euw. 2. File version : 2. File type : zip. File language : German, English, Spanish, French, Italian, Japanese, Korea, Russian, Simplified Chinese. File size : 9. 7,4. KBCanon Singapore Pte. Ltd. makes no guarantees of any kind with regard to any programs, files, drivers or any other materials contained on or downloaded from this, or any other, Canon software site. All such programs, files, drivers and other materials are supplied "as is". Canon disclaims all warranties, expressed or implied, including, without limitation, implied warranties of merchantability, fitness for a particular purpose and non- infringement. Canon Singapore Pte. Ltd. shall not be held liable for errors contained herein, or lost profits, lost opportunities consequential or incidentals damages incurred as a result of acting on information, or the operations of any software, included in this software site. Export restriction: You agree not to send or bring the Software or its documentation out of the country where you originally obtained it to other countries without any required authorization of the applicable governments. You agree to comply with all export laws and restrictions and regulations of the country(ies) involved, as well as with the U. S. Export Administration Regulations ("EAR"), and not to export or re- export, directly or indirectly, the Software in violation of such laws, restrictions and regulations, or without all necessary approvals. ![]()
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Upcoming Class Dates & Times: A Summary Of Our VMware Training Course Our interactive course that looks at the installation, configuration and administration of. ![]() ![]() Free. NAS 9. 1. 0 on VMware ESXi 6. Guide. This is a guide which will install Free. NAS 9. 1. 0 under VMware ESXi and then using ZFS share the storage back to VMware. This is roughly based on Napp- It’s All- In- One design, except that it uses Free. NAS instead of Omin. OS. Disclaimer: I should note that Free. NAS does not officially support running virtualized in production environments. If you run into any problems and ask for help on the Free. NAS forums, I have no doubt that Cyberjock will respond with “So, you want to lose all your data?” So, with that disclaimer aside let’s get going: Update: Josh Paetzel wrote a post on. Virtualizing Free. NASso this is somewhat “official” now. I would still exercise caution. Update 2: This guide was originally written for Free. NAS 9. 3, I’ve updated it for Free. NAS 9. 1. 0. Also, I believe Avago LSI P2. I’ve removed my warning on using P2. Added sections 7. Resource reservations) and 1. Get proper hardware. Example 1: Supermicro 2. U Build. Super. Micro X1. SL7- F (which has a built in LSI2. HBA). Xeon E3- 1. ECC Memory. 6 hotswap bays with 2. TB HGST HDDs (I use RAID- Z2)4 2. Intel DC S3. 70. 0’s for SLOG / ZIL, and 2 drives for installing Free. NAS (mirrored)Example 2: Mini- ITX Datacenter in a Box Build. X1. 0SDV- F (build in Xeon D- 1. ECC Memory. IBM 1. LSI 9. 22. 0- 8i HBA4 hotswap bays with 2. TB HGST HDDs (I use RAID- Z)2 Intel DC S3. SLOG / ZIL, and one to boot ESXi and install Free. NAS to. Hard drives. See info on my Hard Drives for ZFS post. The LSI2. 30. 8/M1. I like do to two DC S3. SLOG device and then do a RAID- Z2 of spinners on the other 6 slots. Also get one (preferably two for a mirror) drives that you will plug into the SATA ports (not on the LSI controller) for the local ESXi data store. I’m using DC S3. 70. I have, but this doesn’t need to be fast storage, it’s just to put Free. NAS on. 2. Flash HBA to IT Firmware. As of Free. NAS 9. IT mode P2. 0 (looks like it’s P2. I strongly suggest pulling all drives before flashing. LSI 2. IT firmware for Supermicro. Here’s instructions to flash the firmware: http: //hardforum. Supermicro firmware: ftp: //ftp. Driver/SAS/LSI/2. Firmware/IT/For IBM M1. LSI Avago 9. 22. 0- 8i. Instructions for flashing firmware: https: //forums. LSI / Avago Firmware: http: //www. If you already have the card passed through to Free. NAS via VT- d (steps 6- 8) you can actually flash the card from Free. NAS using the sas. IT mode so I’m just upgrading it). Package_P2. 0_IR_IT_FW_BIOS_for_MSDOS_Windows. Firmware/HBA_9. 21. IT/2. 11. 8it. bin - b sasbios_rel/mptsas. LSI Corporation SAS2 Flash Utility. Version 1. 6. 0. 0. Copyright (c) 2. 00. LSI Corporation. All rights reserved. Advanced Mode Set. Adapter Selected is a LSI SAS: SAS2. B2). Executing Operation: Flash Firmware Image. Firmware Image has a Valid Checksum. Firmware Version 2. Firmware Image compatible with Controller. Valid NVDATA Image found. NVDATA Version 1. Checking for a compatible NVData image.. NVDATA Device ID and Chip Revision match verified. NVDATA Versions Compatible. Valid Initialization Image verified. Valid Boot. Loader Image verified. Beginning Firmware Download.. Firmware Download Successful. Verifying Download.. Firmware Flash Successful. Resetting Adapter.. Package_P2. 0_IR_IT_FW_BIOS_for_MSDOS_Windows. Firmware/HBA_9. 21. IT/2. 11. 8it. bin - b sasbios_rel/mptsas. LSI Corporation SAS2 Flash Utility. Version. 16. 0. 0. Copyright(c)2. 00. LSI Corporation. All rights reserved. Advanced Mode Set. Adapter Selected isa. LSI SAS: SAS2. 00. B2)Executing Operation: Flash Firmware Image. Firmware Image hasa. Valid Checksum. Firmware Version. Firmware Image compatible with Controller. Valid NVDATA Image found. NVDATA Version. 14. Checking foracompatible NVData image.. NVDATA Device IDand. Chip Revision match verified. NVDATA Versions Compatible. Valid Initialization Image verified. Valid Boot. Loader Image verified. Beginning Firmware Download.. Firmware Download Successful. Verifying Download.. Firmware Flash Successful. Resetting Adapter..(Wait a few minutes, at this point Free. NAS finally crashed. Poweroff. Free. NAS, and then reboot VMware)Warning on P2. Some earlier versions of the P2. P2. 0. 0. 0. 0. 4. If you can’t P2. 0 in aversion later than P2. P1. 9 or P1. 6. 3. Optional: Over- provision ZIL / SLOG SSDs. If you’re going to use an SSD for SLOG you can over- provision them. You can boot into an Ubuntu Live. CD and use hdparm, instructions are here: https: //www. SSD_Over- provisioning_using_hdparm You can also do this after after VMware is installed by passing the LSI controller to an Ubuntu VM (Free. NAS doesn’t have hdparm). I usually over- provision down to 8. GB. Update 2. 01. But you may want to only go to 2. GB depending on your setup! One of my colleagues discovered 8. GB over- provisioning wasn’t even maxing out 1. Gb network (remember, every write to VMware is a sync so it hits the ZIL no matter what) with 2 x 1. Gb fiber lagged connections between VMware and Free. NAS. This was on an HGST 8. Intel DC S3. 70. 0… and it wasn’t virtualized setup. But thought I’d mention it here. Install VMware ESXi 6. The free version of the hypervisor is here. I usually install it to a USB drive plugged into the motherboard’s internal header. Under configuration, storage, click add storage. Choose one (or two) of the local storage disks plugged into your SATA ports (do not add a disk on your LSI controller). Create a Virtual Storage Network. For this example my VMware management IP is 1. VMware Storage Network ip is 1. Free. NAS Storage Network IP is 1. Create a virtual storage network with jumbo frames enabled. VMware, Configuration, Add Networking. Virtual Machine…Create a standard switch (uncheck any physical adapters). Add Networking again, VMKernel, VMKernel… Select v. Switch. 1 (which you just created in the previous step), give it a network different than your main network. I use 1. 0. 5. 5. IP and 2. 55. 2. 55. Some people are having trouble with an MTU of 9. I suggest leaving the MTU at 1. MTU of 9. 00. 0. Also, if you run into networking issues look at disabling TSO offloading (see comments). Under v. Switch. 1 go to Properties, select v. Switch, Edit, change the MTU to 9. Answer yes to the no active NICs warning. Then select the Storage Kernel port, edit, and set the MTU to 9. Configure the LSI 2. Passthrough (VT- d). Configuration, Advanced Settings, Configure Passthrough. Mark the LSI2. 30. You must have VT- d enabled in the BIOS for this to work so if it won’t let you for some reason check your BIOS settings. Reboot VMware. 7. Create the Free. NAS VM. Download the Free. NAS ISO from http: //www. Create a new VM, choose custom, put it on one of the drives on the SATA ports, Virtual Machine version 1. Guest OS type is Free. BSD 6. 4- bit, 1 socket and 2 cores. Try to give it at least 8. GB of memory. On Networking give it two adapters, the 1st NIC should be assigned to the VM Network, 2nd NIC to the Storage network. Set both to VMXNET3. SCSI controller should be the default, LSI Logic Parallel. Choose Edit the Virtual Machine before completion. If you have a second local drive (not one that you’ll use for your zpool) here you can add a second boot drive for a mirror. Before finishing the creation of the VM click Add, select PCI Devices, and choose the LSI 2. And be sure to go into the CD/DVD drive settings and set it to boot off the Free. NAS iso. Then finish creation of the VM. Free. NAS VM Resource allocation. Also, since Free. NAS will be driving the storage for the rest of VMware, it’s a good idea to make sure it has a higher priority for CPU and Memory than other guests. Edit the virtual machine, under Resources set the CPU Shares to “High” to give Free. NAS a higher priority, then under Memory allocation lock the guest memory so that VMware doesn’t ever borrow from it for memory ballooning. You don’t want VMware to swap out ZFS’s ARC (memory read cache). Install Free. NAS. Boot of the VM, install it to your SATA drive (or two of them to mirror boot). After it’s finished installing reboot. Install VMware Tools. SKIP THIS STEP. As of Free. NAS 9. 1. 0. 1 installing VMware should may no longer be necessary–you can skip step 9 and go to 1. Just leaving this for historical purposes. In VMware right- click the Free. NAS VM, Choose Guest, then Install/Upgrade VMware Tools. You’ll then choose interactive mode. Mount the CD- ROM and copy the VMware install files to Free. NAS. # mkdir /mnt/cdrom. VMware\ Tools /mnt/cdrom/. Free. BSD9. 0- amd. VMware\ Tools /mnt/cdrom/# cp /mnt/cdrom/vmware- freebsd- tools. Free. BSD9. 0- amd. Once installed Navigate to the Web. VMware ESXi 5. 0 Update 3 Release Notes ESXi 5. Update 3 | 1. 7 OCT 2. Build 1. 31. 11. 75 Last updated: 3. OCT 2. 01. 3What's in the Release Notes. These release notes cover the following topics. What's New. The following information describes some of the enhancements available in this release of VMware ESXi: Resolved Issues - This release delivers a number of bug fixes that have been documented in the Resolved Issues section. Earlier Releases of ESXi 5. Features and known issues of ESXi 5. To view release notes for earlier releases of ESXi 5. Internationalization. VMware v. Sphere 5. Update 3 is available in the following languages: English. French. German. Japanese. Korean. Simplified Chinesev. Sphere Client Locale Forcing Mode. With v. Sphere 5. Update 3, you can configure the VMware v. Sphere Client to provide the interface text in English even when the machine on which it is running is not English. You can set this configuration for the duration of a single session by supplying a command- line switch. This configuration applies to the interface text and does not affect other locale- related settings such as date and time or numeric formatting. The following v. Sphere Client command causes the individual session to appear in English: vpx. Client - locale en_USCompatibility and Installation. ESXi, v. Center Server, and v. Sphere Client Version Compatibility. The VMware Product Interoperability Matrix provides details about the compatibility of current and previous versions of VMware v. Sphere components, including ESXi, VMware v. Center Server, the v. Sphere Client, and optional VMware products. In addition, check this site for information about supported management and backup agents before installing ESXi or v. Center Server. The v. Sphere Web Client and the v. Sphere Client are packaged with the v. Center Server and modules ZIP file. You can install one or both clients from the VMware v. Center™ Installer wizard. ESXi, v. Center Server, and VDDK Compatibility. Virtual Disk Development Kit (VDDK) 5. ESXi 5. 0 Update 3 and v. Center Server 5. 0 Update 3 releases. For more information about VDDK, see http: //www. Hardware Compatibility for ESXi. To determine which processors, storage devices, SAN arrays, and I/O devices are compatible. Sphere 5. 0 Update 3, use the ESXi 5. Update 3 information in the. VMware Compatibility Guide. Upgrades and Installations for supported CPUs. Sphere 5. 0 Update 3 supports only CPUs that have LAHF and SAHF CPU instruction sets. During an installation or upgrade, the installer checks the compatibility of the host CPU with v. Sphere 5. 0 Update 3. For CPU support, see the. VMware Compatibility Guide. Guest Operating System Compatibility for ESXi. To determine which guest operating systems are compatible with v. Sphere 5. 0 Update 3, use the ESXi 5. Update 3 information in the VMware Compatibility Guide. Virtual Machine Compatibility for ESXi. Virtual machines with virtual hardware versions 4. ESXi 5. 0 Update 3. Hardware version 3 is no longer supported. To use hardware version 3 virtual machines on ESXi 5. Update 3, upgrade virtual hardware. See the v. Sphere Upgrade documentation. Installation Notes for This Release. Read the v. Sphere Installation and Setup documentation for step- by- step guidance on installing and configuring ESXi and v. Center Server. After successful installation, you must perform some licensing, networking, and security configuration. For information about these configuration tasks, see the following guides in the v. Sphere documentation. Migrating Third- Party Solutions. ESX/ESXi hosts might contain third- party software, such as Cisco Nexus 1. V VEMs or EMC Power. Path modules. The ESXi 5. ESX/ESXi 4. x so that customized third- party software packages (VIBs) cannot be migrated when you upgrade from ESX/ESXi 4. ESXi 5. 0 and later. When you upgrade a 4. VIBs that are not in the upgrade ISO, you can proceed with the upgrade but will receive an error message listing the missing VIBs. To upgrade or migrate such hosts successfully, you must use Image Builder to create a custom ESXi ISO image that includes the missing VIBs. To upgrade without including the third- party software, use the Force. Migrate option or select the option to remove third- party software modules during the remediation process in v. Sphere Update Manager. For information about how to use Image Builder to make a custom ISO, see the v. Sphere Installation and Setup documentation. For information about upgrading with third- party customizations, see the v. Sphere Upgrade and Installing and Administering VMware v. Sphere Update Manager documentation. For. information about upgrading with v. Sphere Update Manager, see the v. Sphere Upgrade and Installing and Administering VMware v. Sphere Update Manager documentation. L3- routed NFS Storage Access. Sphere 5. 0 Update 3 supports L3 routed NFS storage access when your environment meets the following conditions: Use Cisco's Hot Standby Router Protocol (HSRP) in IP Router. If you are using a non- Cisco router, be sure to use Virtual Router Redundancy Protocol (VRRP) instead. Use Quality of Service (Qo. S) to prioritize NFS L3 traffic on networks with limited bandwidths, or on networks that experience congestion. See your router documentation for details. Follow Routed NFS L3 best practices recommended by your storage vendor. Contact your storage vendor for details. Disable Network I/O Resource Management (Net. IORM)If you are planning to use systems with top- of- rack switches or switch- dependent I/O device partitioning, contact your system vendor for compatibility and support. In an L3 environment the following additional restrictions are applicable. The environment does not support VMware Site Recovery Manager. The environment supports only NFS protocol. Do not use other storage protocols such as FCo. E over the same physical network. The NFS traffic in this environment does not support IPv. The NFS traffic in this environment can be routed only over a LAN. Other environments such as WAN are not supported. The environment does not support Distributed Virtual Switch (DVS). Upgrades for This Release. For instructions about how to upgrade v. Center Server and ESXi hosts, see the v. Sphere Upgrade documentation. Upgrading VMware Tools. VMware ESXi 5. 0 Update 3 contains the latest version of VMware Tools. VMware Tools is a suite of utilities that enhances the performance of the virtual machine's guest operating system. Refer to the VMware Tools Resolved Issues for a list of issues resolved in this release of ESX related to VMware Tools. To determine an installed VMware Tools version, see Verifying a VMware Tools build version (KB 1. ESX/ESXi Upgrades. You can upgrade ESX/ESXi hosts to ESXi 5. Update 3 in several ways. Sphere Update Manager. If your site uses v. Center Server, use v. Sphere Update Manager to perform an orchestrated host upgrade or an orchestrated virtual machine upgrade from ESX/ESXi 4. ESXi 5. 0. See the instructions in the v. Sphere Upgrade documentation, or for complete documentation about v. Sphere Update Manager, see the Installing and Administering VMware v. Sphere Update Manager documentation. Upgrade interactively using an ESXi installer ISO image on CD- ROM or DVD. You can run the ESXi 5. Update 3 installer from a CD- ROM or DVD drive to perform an interactive upgrade. This method is appropriate for upgrading a small number of hosts. Perform a scripted upgrade. You can upgrade or migrate from ESXi/ESX 4. ESXi 5. 0 Update 3 by running an update script, which provides an efficient, unattended upgrade. Scripted upgrades also provide an efficient way to deploy multiple hosts. You can use a script to upgrade ESXi from a CD- ROM or DVD drive, or by PXE- booting the installer. ESXCLI: You can update and apply patches to ESXi 5. You cannot use esxcli to upgrade ESX/ESXi 4. ESXi 5. 0 Update 3. Supported Upgrade Paths for Upgrade to ESXi 5. Update 3 : Upgrade Deliverables. Supported Upgrade Tools. Supported Upgrade Paths to ESXi 5. Update 3 ESX 4. 0: Includes. ESX 4. 0 Update 1. ESX4. 0 Update 2 ESX4. Update 3. ESX 4. 0 Update 4 ESXi 4. Includes. ESXi 4. Update 1. ESXi 4. Update 2 ESXi 4. 0 Update 3. ESXi 4. 0 Update 4 ESX 4. Includes. ESX 4. 1 Update 1. ESX 4. 1 Update 2. ESX 4. 1 Update 3 ESXi. Includes. ESXi 4. Update 1. ESXi 4. Update 2. ESXi 4. Update 3 ESXi 5. 0: Includes. ESXi 5. 0 Update 1. ESXi 5. 0 Update 2 VMware- VMvisor- Installer- 5. VMware v. Center Update Manager CD Upgrade Scripted Upgrade Yes. Yes. Yes. Yes. Yes*update- from- esxi. VMware v. Center Update Manager. Yahoo奇摩購物中心- 數十萬件商品8. ![]() 數十萬件商品8H急速配!購衷心會員刷Yahoo聯名卡筆筆回饋最高6%!24小時客服全年無休,APP訂單詢問30分鐘速回覆!. Usage Statistics for communitygaze.com Summary Period: June 2017 - Search String Generated 01-Jul-2017 02:11 PDT. ![]() TheINQUIRER publishes daily news, reviews on the latest gadgets and devices, and INQdepth articles for tech buffs and hobbyists. ADVANCE Xi - 125, Køb en XI 125 for kun kr. 500 /md i 20 måneder uden udgift for dig. ( ÅOP 0%). Det tager 5 minutter at oprette kontrakten. Computer-aided drug discovery/design methods have played a major role in the development of therapeutically important small molecules for over three decades. These. Artificial intelligence (AI, also machine intelligence, MI) is intelligence exhibited by machines, rather than humans or other animals (natural intelligence, NI). The online version of Environmental Modelling & Software at ScienceDirect.com, the world's leading platform for high quality peer-reviewed full-text journals. Genome engineering using the CRISPR- Cas. Abstract. Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA- guided Cas. CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in eukaryotic cells by simply specifying a 2. RNA. Here we describe a set of tools for Cas. NHEJ) or homology- directed repair (HDR) in mammalian cells, as well as generation of modified cell lines for downstream functional studies. Cas. 9 nickase mutant with paired guide RNAs. This protocol provides experimentally derived guidelines for the selection of target sites, evaluation of cleavage efficiency and analysis of off- target activity. Beginning with target design, gene modifications can be achieved within as little as 1–2 weeks, and modified clonal cell lines can be derived within 2–3 weeks. INTRODUCTIONThe ability to engineer biological systems and organisms holds enormous potential for applications across basic science, medicine and biotechnology. Programmable sequence- specific endonucleases that facilitate precise editing of endogenous genomic loci are now enabling systematic interrogation of genetic elements and causal genetic variations. A number of genome editing technologies have emerged in recent years, including zinc- finger nucleases (ZFNs)7–1. TALENs)1. 0–1. 7 and the RNA- guided CRISPR- Cas nuclease system. The first two technologies use a strategy of tethering endonuclease catalytic domains to modular DNA- binding proteins for inducing targeted DNA double- stranded breaks (DSBs) at specific genomic loci. By contrast, Cas. RNAs through Watson- Crick base pairing with target DNA2. Fig. 1), representing a system that is markedly easier to design, highly specific, efficient and well- suited for high- throughput and multiplexed gene editing for a variety of cell types and organisms. Schematic of the RNA- guided Cas. The Cas. 9 nuclease from S. DNA (shown for example is the human EMX1 locus) by an sg. RNA consisting of a 2. The guide sequence.. Precise genome editing using engineered nucleases. Similarly to ZFNs and TALENs, Cas. DSB at a target genomic locus. Upon cleavage by Cas. DNA damage repair (Fig. NHEJ or the high- fidelity HDR pathway, both of which can be used to achieve a desired editing outcome. In the absence of a repair template, DSBs are re- ligated through the NHEJ process, which leaves scars in the form of insertion/deletion (indel) mutations. NHEJ can be harnessed to mediate gene knockouts, as indels occurring within a coding exon can lead to frameshift mutations and premature stop codons. Multiple DSBs can additionally be exploited to mediate larger deletions in the genome. DSB repair promotes gene editing. DSBs induced by Cas. In the error- prone NHEJ pathway, the ends of a DSB are processed by endogenous DNA repair machinery and rejoined, which can result in random indel mutations.. HDR is an alternative major DNA repair pathway. Although HDR typically occurs at lower and substantially more variable frequencies than NHEJ, it can be leveraged to generate precise, defined modifications at a target locus in the presence of an exogenously introduced repair template. The repair template can either be in the form of conventional double- stranded DNA targeting constructs with homology arms flanking the insertion sequence, or single- stranded DNA oligonucleotides (ss. ODNs). The latter provides an effective and simple method for making small edits in the genome, such as the introduction of single- nucleotide mutations for probing causal genetic variations. Unlike NHEJ, HDR is generally active only in dividing cells, and its efficiency can vary widely depending on the cell type and state, as well as the genomic locus and repair template. Cas. 9: an RNA- guided nuclease for genome editing. CRISPR- Cas is a microbial adaptive immune system that uses RNA- guided nucleases to cleave foreign genetic elements. Three types (I–III) of CRISPR systems have been identified across a wide range of bacterial and archaeal hosts, wherein each system comprises a cluster of CRISPR- associated (Cas) genes, noncoding RNAs and a distinctive array of repetitive elements (direct repeats). These repeats are interspaced by short variable sequences. DNA targets known as protospacers, and together they constitute the CRISPR RNA (cr. RNA) array. Within the DNA target, each protospacer is always associated with a protospacer adjacent motif (PAM), which can vary depending on the specific CRISPR system. The Type II CRISPR system is one of the best characterized. Cas. 9, the cr. RNA array that encodes the guide RNAs and a required auxiliary trans- activating cr. RNA (tracr. RNA) that facilitates the processing of the cr. RNA array into discrete units. Each cr. RNA unit then contains a 2. Cas. 9 to a 2. 0- bp DNA target via Watson- Crick base pairing (Fig. In the CRISPR- Cas system derived from Streptococcus pyogenes (which is the system used in this protocol), the target DNA must immediately precede a 5′- NGG PAM2. Cas. 9 orthologs may have different PAM requirements, such as those of S. NNAGAA2. 2,2. 6 for CRISPR1 and 5′- NGGNG2. CRISPR3) and Neisseria meningiditis (5′- NNNNGATT)3. The RNA- guided nuclease function of CRISPR- Cas is reconstituted in mammalian cells through the heterologous expression of human codon–optimized Cas. RNA components. 22–2. Furthermore, the cr. RNA and tracr. RNA can be fused together to create a chimeric, single- guide RNA (sg. RNA)2. 7 (Fig. 1). Cas. 9 can thus be re- directed toward almost any target of interest in immediate vicinity of the PAM sequence by altering the 2. RNA. Given its ease of implementation and multiplexing capacity, Cas. NHEJ and HDR2. 2–2. Direct injection of sg. RNA and m. RNA encoding Cas. These results hold enormous promise for editing organisms that are otherwise genetically intractable. Cas. 9 nucleases carry out strand- specific cleavage by using the conserved HNH and Ruv. C nuclease domains, which can be mutated and exploited for additional function. An aspartate- to- alanine (D1. A) mutation in the Ruv. C catalytic domain. Cas. 9 nickase mutant (Cas. DNA to yield single- stranded breaks, and the subsequent preferential repair through HDR2. DSBs. Appropriately offset sg. RNA pairs can guide Cas. DSB, thus effectively increasing the specificity of target recognition. In addition, a Cas. DNA- cleaving catalytic residues mutated has been adapted to enable transcriptional regulation in Escherichia coli. Cas. 9 for diverse applications, such as recruitment of fluorescent protein labels or chromatin- modifying enzymes to specific genomic loci for reporting or modulating gene function. Here we explain in detail how to use a human codon–optimized, nuclear localization sequence- flanked wild- type (WT) Cas. Cas. 9 nickase to facilitate eukaryotic gene editing. We describe considerations for designing the 2. RNAs and finally the use of the Cas. NHEJ- and HDR- based genome modifications in human embryonic kidney (HEK 2. FT) and human stem cell (HUES9) lines (Fig. The Cas. 9 system can similarly be applied to other cell types and organisms, including humans. Drosophila. 46 and Caenorhabditis elegans. Timeline and overview of experiments. Steps for reagent design, construction, validation and cell line expansion are depicted. Custom sg. RNAs (light blue bars) for each target, as well as genotyping primers, are designed in silico via the CRISPR Design.. Comparison with other genome editing technologies. As with other designer nuclease technologies such as ZFNs and TALENs, Cas. DNA DSBs at specific loci of interest in the mammalian genome and stimulate genome editing via NHEJ or HDR. Cas. 9 offers several potential advantages over ZFNs and TALENs, including the ease of customization, higher targeting efficiency and the ability to facilitate multiplex genome editing. As custom ZFNs are often difficult to engineer, we will primarily compare Cas. TALEN.• Ease of customization Cas. DNA sequences by simply purchasing a pair of oligos encoding the 2. In contrast, retargeting of TALEN for a new DNA sequence requires the construction of two new TALEN genes. Although a variety of protocols exist for TALEN construction. TALENs.• Cleavage pattern WT S. Cas. 9 (Sp. Cas. 9) is known to make a blunt cut between the 1. PAM)2. 7. Mutating catalytic residues in either the Ruv. C or the HNH nuclease domain of Sp. Cas. 9 converts the enzyme into a DNA nicking enzyme. In contrast, TALENs cleave nonspecifically in the 1. TALEN monomer- binding sites. Editing efficiency Sp. ![]() Indians Win Record- Breaking 2. Consecutive Game. The Indians will apparently never lose again. They won their 2. Detroit Tigers. The win ties the streak held by the 1. An article should begin with an introductory lead section, which should not contain section headings (see Wikipedia:Manual of Style/Lead section). Cubs, and gives Cleveland sole ownership of the AL win streak record, which the A’s have held since 2. They are now five games away from tying the 2. New York Giants, although that lossless streak comes with its own qualifications: The Giants had a rain- shortened 1- 1 game go down as a tie in the middle of that streak, so they didn’t technically win 2. If Cleveland is going to reach 2. 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